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96 Wells

Additional Information

Name HCRP1 ELISA Kit (Human) (GWB-KBBDA1)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.049 ng/mL
Sample Type Serum, plasma, tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol HCRP1
Gene id 137492
Alias Symbols ESCRT-I complex subunit VPS37A, FLJ32642, FLJ42616, HCRP1, Hepatocellular carcinoma-related protein 1, hVps37A, PQBP2, Vacuolar protein sorting-associated protein 37A
Swissprot ID Q8NEZ2
Protein Name Vacuolar protein sorting-associated protein 37A
Description of Target This gene belongs to the VPS37 family, and encodes a component of the ESCRT-I (endosomal sorting complex required for transport I) protein complex, required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular b
Kit Principle Genway Biotech HCRP1 ELISA Kit (Human) (GWB-KBBDA1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HCRP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HCRP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HCRP1 captured in the well.
Kit Component
Anti-HCRP1 Microplate96 Wells (12 x 8 Well Strips)
HCRP1 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X HCRP1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001138624.1
Nucleotide Accession Num NM_001145152.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBDA1
Species Reactivity Human