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96 Wells
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Additional Information

Name HDAC5 ELISA Kit (Human) (GWB-KBBUR1)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.113 ng/mL
Sample Type tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol HDAC5
Gene id 10014
Alias Symbols Antigen NY-CO-9, FLJ90614, HD5, Histone deacetylase 5, KIAA0600, NY-CO-9
Swissprot ID Q9UQL6
Protein Name Histone deacetylase 5
Description of Target Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progr
Kit Principle Genway Biotech HDAC5 ELISA Kit (Human) (GWB-KBBUR1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HDAC5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HDAC5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HDAC5 captured in the well.
Kit Component
Anti-HDAC5 Microplate96 Wells (12 x 8 Well Strips)
HDAC5 Lyophilized Standard2 x 20 ng
100X Biotinylated HDAC5 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001015053
Nucleotide Accession Num NM_001015053
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBUR1
Species Reactivity Human