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96 Wells
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Additional Information

Name HDGF ELISA Kit (Human) (GWB-KBBOX6)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.059 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol HDGF
Gene id 3068
Alias Symbols DKFZp686J1764, FLJ96580, Hepatoma-derived growth factor, High mobility group protein 1-like 2, HMG1L2, HMG-1L2
Swissprot ID P51858
Protein Name hepatoma-derived growth factor
Description of Target Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.
Kit Principle Genway Biotech HDGF ELISA Kit (Human) (GWB-KBBOX6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HDGF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HDGF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HDGF captured in the well.
Kit Component
Anti-Casp9 Microplate96 Wells (12 x 8 Well Strips)
Casp9 Lyophilized Standard2 x 10 ng
100X Biotinylated Casp9 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001119522
Nucleotide Accession Num NM_001126050
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)79-90%78-89%88-104%80-101%
EDTA Plasma (n=5)97-105%80-101%78-91%82-96%
Heparin Plasma (n=5)79-103%89-104%85-94%84-95%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)97-104101
EDTA Plasma (n=5)98-105101
Heparin Plasma (n=5)93-10197
Datasheets/Manuals Printable datasheet for GWB-KBBOX6
Species Reactivity Human