Histatin 5

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Sequence: {ASP} {SER} {HIS} {ALA} {LYS} {ARG} {HIS} {HIS} {GLY} {TYR} {LYS} {ARG} {LYS} {PHE} {HIS} {GLU} {LYS} {HIS} {HIS} {SER} {HIS} {ARG} {GLY} {TYR}

Size: 0.5 mg Formula: C133H195N51O33 MW: 3036.29 Histatin 5 is a human basic salivary peptide with strong fungicidal properties in vitro. To elucidate the mechanism of action. the effect of Histatin 5 on the viability of Candida albicans cells was studied in relation to its membrane perturbing properties. It was found that both the killing activity and the membrane perturbing activity. studied by the influx of a DNA-specific marker propidium iodide. were inhibited by high salt conditions and by metabolic inhibitors. like sodium azide.

Function: Histatins are salivary proteins that are considered to be major precursors of the protective proteinaceous structure on tooth surfaces (enamel pellicle). In addition, histatins exhibit antibacterial and antifungal activities. Histatin-3 1/24 (Histatin 5) is especially effective against C.albicans and C.neoformans, and inhibits Lys-gingipain and Arg-gingipain (rgpB) from P.gingivalis. In addition, histatin-3 1/24 is a potent inhibitor of metalloproteinases MMP2 and MMP9.

Subunit: Histatin-3 1/24 is a homodimer. Histatin-3 and histatin-3 1/24 5 interact with yeast SSA1 and SSA2 proteins.

Subcellular Location: Secreted protein. Secreted by serous acinar and demilune cells.

Ptm: 24 proteolytic products are found in saliva.

Polymorphism: There are two alleles of HIS2, HIS2(1) (shown here) and HIS2(2) that codes for the variant histatin-3-2 found primarily and in high frequencies in black populations.

Similarity: To histatin 1.

Summary: The primary protein encoded by HTN3 is histatin 3 Histatins are a family of small, histidine-rich, salivary proteins, encoded by at least two loci (HTN3 and HTN1). Post-translational proteolyitic processing results in many histatins: e.g., histatins 4-6 are derived from histatin 3 by proteolysis. Histatins are believed to have important non-immunological, anti-microbial function in the oral cavity. [1] Bruno,L.S., Li,X., Wang,L., Soares,R.V., Siqueira,C.C., Oppenheim,F.G., Troxler,R.F. and Offner,G.D.
Two-hybrid analysis of human salivary mucin MUC7 interactions
[2] Castagnola,M., Inzitari,R., Rossetti,D.V., Olmi,C., Cabras,T., Piras,V., Nicolussi,P., Sanna,M.T., Pellegrini,M., Giardina,B. and, et al.
A cascade of 24 histatins (histatin 3 fragments) in human saliva. Suggestions for a pre-secretory sequential cleavage pathway
[3] Li,X.S., Reddy,M.S., Baev,D. and Edgerton,M., et al.
Candida albicans Ssa1/2p is the cell envelope binding protein for human salivary histatin 5
[4] Baev,D., Li,X.S., Dong,J., Keng,P. and Edgerton,M., et al.
Human salivary histatin 5 causes disordered volume regulation and cell cycle arrest in Candida albicans
[5] Gyurko,C., Lendenmann,U., Helmerhorst,E.J., Troxler,R.F. and Oppenheim,F.G.
Killing of Candida albicans by histatin 5: cellular uptake and energy requirement
[6] Sabatini L.M., Azen E.A.
Histatins, a family of salivary histidine-rich proteins, are encoded by at least two loci (HIS1 and HIS2).
[7] Dickinson D.P., Ridall A.L., Levine M.J.
Human submandibular gland statherin and basic histidine-rich peptide are encoded by highly abundant mRNA\'s derived from a common ancestral sequence.
[8] Vanderspek J.C., Offner G.D., Troxler R.F., Oppenheim F.G.
Molecular cloning of human submandibular histatins.
[9] Chen Z.W.
Nucleotide sequence analysis of the human salivary protein genes HIS1 and HIS2, and evolution of the STATH/HIS gene family.
[10] Sabatini L.M., Azen E.A.
Two coding change mutations in the HIS2(2) allele characterize the salivary histatin 3-2 protein variant.

Additional Information

Name Histatin 5
Related Product Names Histidine-rich protein 3; PB; Basic histidine-rich protein; Hst HIS2Histatin 5HTN3
Intended Use Research Use Only
NCBI Acc Number NP_000191.1
GI Number 4557653
Purity > 95%
Stability 6-12 months at -20 degree C.
Storage Store at -20C
Molecular Weight 6149
Sequence Length 51
Swiss Prot Number P15516