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96 Wells
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Additional Information

Name HPD ELISA Kit (Human) (GWB-KBBLN8)
Kit Range 0.78 - 50 ng/mL
Sensitivity < 0.32 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol HPD
Gene id 3242
Alias Symbols 4HPPD, 4-HPPD, 4-hydroxyphenylpyruvic acid oxidase, GLOD3, HPPDase, HPPDASE, PPD
Swissprot ID P32754
Protein Name 4-hydroxyphenylpyruvate dioxygenase
Description of Target Key enzyme in the degradation of tyrosine.
Kit Principle Genway Biotech HPD ELISA Kit (Human) (GWB-KBBLN8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HPD has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HPD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HPD captured in the well.
Kit Component
Anti-Eng Microplate96 Wells (12 x 8 Well Strips)
Eng Lyophilized Standard2 x 50 ng
100X Biotinylated Eng Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001165464
Nucleotide Accession Num NM_001171993
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)95-104%84-95%78-99%88-105%
EDTA Plasma (n=5)78-90%90-97%95-105%83-97%
Heparin Plasma (n=5)78-104%93-105%80-92%85-99%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)91-9995
EDTA Plasma (n=5)90-9995
Heparin Plasma (n=5)88-9592
Datasheets/Manuals Printable datasheet for GWB-KBBLN8
Species Reactivity Human