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Hpd ELISA Kit (Rat) (GWB-KBBIB6)

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96 Wells
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Additional Information

Name Hpd ELISA Kit (Rat) (GWB-KBBIB6)
Kit Range 3.12 - 200 ng/mL
Sensitivity < 1.35 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol HPD
Gene id 29531
Alias Symbols 4HPPD, 4-hydroxyphenylpyruvic acid oxidase, F Alloantigen, F protein, HPD, HPPDase
Swissprot ID P32755
Protein Name 4-hydroxyphenylpyruvate dioxygenase
Description of Target Key enzyme in the degradation of tyrosine.
Kit Principle Genway Biotech Hpd ELISA Kit (Rat) (GWB-KBBIB6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hpd has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hpd is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hpd captured in the well.
Kit Component
Anti-Hpd Microplate96 Wells (12 x 8 Well Strips)
Hpd Lyophilized Standard2 x 200 ng
100X Biotinylated Hpd Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_058929
Nucleotide Accession Num NM_017233
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)85-97%84-93%89-98%83-90%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Datasheets/Manuals Printable datasheet for GWB-KBBIB6
Species Reactivity Rat