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96 Wells
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Additional Information

Name HRH1 ELISA Kit (Human) (GWB-KBBIZ3)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.114 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol HRH1
Gene id 3269
Alias Symbols H1R, H1-R, HH1R, hisH1, Histamine H1 receptor
Swissprot ID P35367
Protein Name Histamine H1 receptor
Description of Target In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neuro
Kit Principle Genway Biotech HRH1 ELISA Kit (Human) (GWB-KBBIZ3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HRH1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HRH1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HRH1 captured in the well.
Kit Component
Anti-HRH1 Microplate96 Wells (12 x 8 Well Strips)
HRH1 Lyophilized Standard2 x 20 ng
100X Biotinylated HRH1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000852
Nucleotide Accession Num NM_000861
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBIZ3
Species Reactivity Human