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96 Wells
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Additional Information

Name HRH1 ELISA Kit (Rat) (GWB-KBBFZ4)
Kit Range 0.156-10 ng/mL
Sensitivity <0.067ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol HRH1
Gene id 24448
Alias Symbols H1R, HH1R, Histamine H1 receptor
Swissprot ID P31390
Protein Name Histamine H1 receptor
Description of Target Activates nitric oxide synthase via g-protein coupled signaling pathways, plays a role in interneuronal coupling
Kit Principle Genway Biotech HRH1 ELISA Kit (Rat) (GWB-KBBFZ4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HRH1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HRH1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HRH1 captured in the well.
Kit Component
Anti-HRH1 Microplate96 Wells (12 x 8 Well Strips)
HRH1 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X HRH1 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_058714.1
Nucleotide Accession Num NM_017018.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBFZ4
Species Reactivity Rat