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96 Wells
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Additional Information

Name HS2ST1 ELISA Kit (Human) (GWB-KBBHO1)
Description of Target Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. This gene encodes a member of the heparan sulfate biosynthetic enzyme family that trans
Kit Range 0.156-10 ng/mL
Sensitivity < 0.052 ng/mL
Sample Type Serum, plasma, tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol HS2ST1
Gene id 9653
Alias Symbols 2OST, 2-OST, 2-O-sulfotransferase, AW214369, Heparan sulfate 2-O-sulfotransferase 1, Hs2st, mKIAA0448
Swissprot ID Q8R3H7
Protein Name Heparan sulfate 2-O-sulfotransferase 1
Kit Principle Genway Biotech HS2ST1 ELISA Kit (Human) (GWB-KBBHO1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HS2ST1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HS2ST1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HS2ST1 captured in the well.
Kit Component
Anti-HS2ST1 Microplate96 Wells (12 x 8 Well Strips)
HS2ST1 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X HS2ST1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_035958.3
Nucleotide Accession Num NM_011828.3
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBHO1
Species Reactivity Human