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Additional Information

Name HS2ST1 ELISA Kit (Human) (GWB-KBBMH5)
Description of Target Catalyzes the transfer of sulfate to the C2-position of selected hexuronic acid residues within the maturing heparan sulfate (HS). 2-O-sulfation within HS, particularly of iduronate residues, is essential for HS to participate in a variety of high-affinit
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.052 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol HS2ST1
Gene id 9653
Alias Symbols 2OST, 2-O-sulfotransferase, dJ604K5.2, FLJ11317, Heparan sulfate 2-O-sulfotransferase 1, HS2ST, KIAA0448, MGC131986
Swissprot ID Q7LGA3
Protein Name heparan sulfate 2-O-sulfotransferase 1
Kit Principle Genway Biotech HS2ST1 ELISA Kit (Human) (GWB-KBBMH5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HS2ST1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HS2ST1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HS2ST1 captured in the well.
Kit Component
Anti-HEMGN Microplate96 Wells (12 x 8 Well Strips)
HEMGN Lyophilized Standard2 x 10 ng
100X Biotinylated HEMGN Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001127964
Nucleotide Accession Num NM_001134492
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBMH5
Species Reactivity Human