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96 Wells
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Additional Information

Name HSPA12B ELISA Kit (Human) (GWB-KBBZL8)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.128 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol HSPA12B
Gene id 116835
Alias Symbols C20orf60, dJ1009E24.2, FLJ32150, Heat shock 70 kDa protein 12B, MGC131912
Swissprot ID Q96MM6
Protein Name heat shock 70 kDa protein 12B
Description of Target The protein encoded by this gene contains an atypical heat shock protein 70 (Hsp70) ATPase domain and is therefore a distant member of the mammalian Hsp70 family. This gene may be involved in susceptibility to atherosclerosis. Alternative splicing results
Kit Principle Genway Biotech HSPA12B ELISA Kit (Human) (GWB-KBBZL8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPA12B has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSPA12B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPA12B captured in the well.
Kit Component
Anti-HIST1H2AB more Microplate96 Wells (12 x 8 Well Strips)
HIST1H2AB more Lyophilized Standard2 x 20 ng
100X Biotinylated HIST1H2AB more Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001184256
Nucleotide Accession Num NM_001197327
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBZL8
Species Reactivity Human