HSPBP1 ELISA Kit (Human) (GWB-KBBIQ6)

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GWB-KBBIQ6

96 Wells
$659.00
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Additional Information

Name HSPBP1 ELISA Kit (Human) (GWB-KBBIQ6)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.118 ng/mL
Sample Type serum, plasma and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol HSPBP1
Gene id 23640
Alias Symbols FES1, Heat shock protein-binding protein 1, Hsp70-binding protein 1, Hsp70-binding protein 2, Hsp70-interacting protein 1, Hsp70-interacting protein 2, HSPBP, HspBP1, HspBP2, PP1845
Swissprot ID Q9NZL4
Protein Name hsp70-binding protein 1
Description of Target Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR.
Kit Principle Genway Biotech HSPBP1 ELISA Kit (Human) (GWB-KBBIQ6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPBP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSPBP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPBP1 captured in the well.
Kit Component
ComponentAmount
Anti-PTPRG Microplate96 Wells (12 x 8 Well Strips)
PTPRG Lyophilized Standard2 x 20 ng
100X Biotinylated PTPRG Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001123578
Nucleotide Accession Num NM_001130106
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)97-105%82-97%87-101%79-96%
EDTA Plasma (n=5)80-99%94-103%94-103%82-97%
Heparin Plasma (n=5)80-104%85-94%84-104%97-104%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)86-10192
EDTA Plasma (n=5)91-10296
Heparin Plasma (n=5)98-105102
Datasheets/Manuals Printable datasheet for GWB-KBBIQ6
Species Reactivity Human