96 Wells
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Additional Information

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Kit Range 0.156-10 ng/mL
Sensitivity <0.055 ng/mL
Sample Type Serum, plasma, tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol HTT
Gene id 29424
Alias Symbols Hd, Hdh
Swissprot ID G3V9P7
Protein Name huntingtin
Description of Target Necessary for normal neuronal development; mutation of human homolog is associated with huntingtons disease [rgd, feb 2006]
Kit Principle Genway Biotech HTT ELISA Kit (Rat) (GWB-KBBVJ2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HTT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HTT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HTT captured in the well.
Kit Component
Anti-HTT Microplate96 Wells (12 x 8 Well Strips)
HTT Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X HTT Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_077333.2
Nucleotide Accession Num NM_024357.3
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBVJ2
Species Reactivity Rat