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Human 4-1BB-R ELISA Kit (GWB-SKR001)

This exact product is now provided by Aviva Systems Biology.
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GWB-SKR001

96 Wells
$479.00
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Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human 4-1BB-R ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human 4-1BB-R proteins within the range of 16-2000 pg/ml. The Human 4-1BBR ELISA is capable of recognizing both recombinant and naturally produced Human 4-1BBR proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human 4-1BBL, AITRL, BAFF, BAFF Receptor, BCMA, CD40 Ligand/TRAP, Fas Receptor, LIGHT, OPG, sOX40L, sRANK (Receptor), sRANKL, TACI, TL-1A, TNF-alpha, TNF-beta, sTNF-Receptor Type I, sTNF-Receptor Type II, sTRAIL/Apo2L, sTRAIL Receptor-1, sTRAIL Receptor-2, TWEAK, TWEAK Receptor

Additional Information

Name Human 4-1BB-R ELISA Kit (GWB-SKR001)
Related Product Names Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor T-cell, antigen 4-1BB homolog, T-cell antigen ILA, CD137 antigen, CDw137, ILA, 4-1BB, MGC2172, 4-1BBR, TNFRSF9
Description of Target The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induc
Kit Range 16-2,000 pg/mL
Sensitivity 16 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol TNFRSF9
Gene Family CD
Gene Fullname tumor necrosis factor receptor superfamily member 9
Gene id 3604
Alias Symbols 4-1BB, 4-1BB ligand receptor, CD137, CDw137, FLJ43501, ILA, MGC2172, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9
Swissprot ID Q07011
Protein Name Tumor necrosis factor receptor superfamily member 9
Kit Principle The Aviva Human BD-1 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human BD-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The captureantibodies coated to the bottom of each well are specific for a particular epitope on Human BD-1 while the user-added detection antibodies bind toepitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (100 ng)
Protein Accession Num NP_001552.2
Nucleotide Accession Num NM_001561.5
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 16-2000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human 4-1BBR ELISA is capable of recognizing both recombinant and naturally produced Human 4-1BBR proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: 4-1BBL, AITRL, BAFF, BAFF Receptor, BCMA, CD40 Ligand/TRAP, Fas Receptor, LIGHT, OPG, sOX40L, sRANK (Receptor), sRANKL, TACI, TL-1A, TNF-α, TNF-β, sTNF-Receptor Type I, sTNF-Receptor Type II, sTRAIL/Apo2L, sTRAIL Receptor-1, sTRAIL Receptor-2, TWEAK, TWEAK Receptor
Application ELISA
GI Number 3604
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Storage 4C/6 Months
Datasheets/Manuals Printable datasheet for GWB-SKR001
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only