Human Albumin ELISA Kit (GWB-64EC6D) (Aviva Cat. No. OKIA00046)

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GWB-64EC6D

Aviva Cat. No.: OKIA00046

40-374-130099

Additional Information

Name Human Albumin ELISA Kit (GWB-64EC6D)
Related Product Names Human Albumin ELISA Kit
Kit Range 6.25 ng/ml - 200 ng/ml
Sensitivity 1.900 ng/ml
Sample Type Serum, plasma and other biological fluids
Gene Symbol ALB
Gene Fullname albumin
Gene id 213
Alias Symbols Albumin, ALB
Swissprot ID P02768
Protein Name Serum albumin
Description of Target Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma,
Kit Principle Genway Biotech Albumin ELISA Kit (Human) (GWB-64EC6D) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Serum albumin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Serum albumin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serum albumin captured in the well.
Kit Component
ComponentAmount
Anti-Human Albumin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human Albumin Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Human
Standard Range Refer To Manual
Product Format In Solution
Specimen/Volumes Refer To Manual
Application ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-Albumin conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-HUMAN ALBUMIN ELISA MICRO PLATE
Anti-Human Albumin coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. HUMAN ALBUMIN CALIBRATOR
The lyophilized Human Albumin calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Human
Kit Size 96 well
Kit Duration 100 Minutes
Datasheets/Manuals Printable datasheet for GWB-64EC6D
Storage Refer to kit instructions for component storage
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only
Additional Information Intended Use: The Albumin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Albumin in biological fluid of Human.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Albuminumin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN ALBUMIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human Albumin.
7. HUMAN ALBUMIN CALIBRATOR
One vial containing a lyophilized Human Albumin calibrator.