Human Alpha 1-Acid Glycoprotein ELISA Kit (GWB-F43C70) (Aviva Cat. No. OKIA00047)

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GWB-F43C70

Aviva Cat. No.: OKIA00047

40-374-130098

Additional Information

Name Human Alpha 1-Acid Glycoprotein ELISA Kit (GWB-F43C70)
Related Product Names Human Alpha 1-Acid Glycoprotein ELISA Kit
Kit Range 5 ng/ml - 320 ng/ml
Sensitivity 1.089 ng/ml
Sample Type Serum, plasma and other biological fluids
Gene Symbol ORM1
Gene Fullname orosomucoid 1
Gene id 5004
Alias Symbols AGP1, ORM1, Alpha-1-acid glycoprotein 1
Swissprot ID P02763
Protein Name Alpha-1-acid glycoprotein 1
Description of Target Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity
Kit Principle Genway Biotech Alpha 1-Acid Glycoprotein ELISA Kit (Human) (GWB-F43C70) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Alpha-1-acid glycoprotein 1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Alpha-1-acid glycoprotein 1 - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alpha-1-acid glycoprotein 1 captured in the well.
Kit Component
ComponentAmount
Anti-Human Alpha 1-Acid Glycoprotein Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human Alpha 1-Acid Glycoprotein Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Human
Standard Range Refer To Manual
Product Format In Solution
Specimen/Volumes Refer To Manual
Application ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-AGP conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-HUMAN AGP ELISA MICRO PLATE
Anti-Human AGP coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. HUMAN AGP CALIBRATOR
The lyophilized Human Alpha 1-Acid Glycoprotein calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Human
Kit Size 96 well
Kit Duration 65 Minutes
Datasheets/Manuals Printable datasheet for GWB-F43C70
Storage Refer to kit instructions for component storage
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only
Additional Information Intended Use: The Alpha 1-Acid Glycoprotein (AGP) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Alpha 1-Acid Glycoprotein in biological fluids in human.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Alpha 1-Acid Glycoprotein antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMANAGP ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human AGP.
7. HUMAN AGP CALIBRATOR
One vial containing a lyophilized Human Alpha 1-Acid Glycoprotein calibrator.