Human Alpha 1-Antitrypsin ELISA Kit (GWB-5428A0)

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GWB-5428A0

40-374-130097

96 Wells
$359.00
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Additional Information

Name Human Alpha 1-Antitrypsin ELISA Kit (GWB-5428A0)
Related Product Names Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Human alpha 1-Antitrypsin ELISA Kit; Alpha-1 protease inhibitor, Alpha-1-antiproteinase, AAT, PI Human alpha 1 Antitrypsin, SERPINA1, PI, AAT ELISA, A1AT, Human Alpha 1-Antitrypsin ELISA Kit
Kit Range 7.81 ng/ml - 500 ng/ml
Sensitivity 1.094 ng/ml
Sample Type Serum, plasma and other biological fluids
Gene Symbol SERPINA1
Gene Fullname serpin family A member 1
Gene id 5265
Alias Symbols SERPINA1, AAT, A1AT, alpha1AT
Swissprot ID P01009
Protein Name Alpha-1-antitrypsin
Description of Target Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in
Kit Principle Genway Biotech Alpha 1-Antitrypsin ELISA Kit (Human) (GWB-5428A0) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Alpha-1-antitrypsin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Alpha-1-antitrypsin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alpha-1-antitrypsin captured in the well.
Kit Component
ComponentAmount
Anti-Human Alpha 1-Antitrypsin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human Alpha 1-Antitrypsin Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Human
Standard Range Refer To Manual
Product Format In Solution
Specimen/Volumes Refer To Manual
Application ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-A1AT conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-HUMAN ALPHA 1-ANTITRYPSIN ELISA MICRO PLATE
Anti-Human A1AT coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. HUMAN ALPHA 1-ANTITRYPSIN CALIBRATOR
The lyophilized Human Alpha 1-Antitrypsin calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Human
Kit Size 96 well
Kit Duration 100 Minutes
Datasheets/Manuals Printable datasheet for GWB-5428A0
Storage Refer to kit instructions for component storage
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only
Additional Information Intended Use: The Alpha 1-Antitrypsin (A1AT) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring A1AT in biological fluid of humans.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Alpha 1-Antitrypsin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN ALPHA 1-ANTITRYPSIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human A1AT.
7. HUMAN ALPHA 1-ANTITRYPSIN CALIBRATOR
One vial containing a lyophilized Human Alpha 1-Antitrypsin calibrator.