Human alpha 1-Microglobulin ELISA Kit (GWB-D2CE63) (Aviva Cat. No. OKIA00051)

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GWB-D2CE63

Aviva Cat. No.: OKIA00051

40-374-130096

Additional Information

Name Human alpha 1-Microglobulin ELISA Kit (GWB-D2CE63)
Related Product Names Human alpha 1-Microglobulin ELISA Kit
Kit Range 12.5 ng/ml - 400 ng/ml
Sensitivity 2.481 ng/ml
Sample Type Serum, plasma and other biological fluids
Gene Symbol AMBP
Gene Fullname alpha-1-microglobulin/bikunin precursor
Gene id 259
Alias Symbols AMBP, Alpha 1-Microglobulin
Swissprot ID P02760
Protein Name Protein AMBP
Description of Target Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
Kit Principle Genway Biotech Alpha 1-Microglobulin ELISA Kit (Human) (GWB-D2CE63) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Protein AMBP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Protein AMBP - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Protein AMBP captured in the well.
Kit Component
ComponentAmount
Anti-Human alpha 1-Microglobulin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human alpha 1-Microglobulin Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Human
Standard Range Refer To Manual
Product Format In Solution
Specimen/Volumes Refer To Manual
Application ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-A1M conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-HUMAN A1M ELISA MICRO PLATE
Anti-Human A1M coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. HUMAN A1M CALIBRATOR
The lyophilized Human Alpha 1 Microglobulin calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 1 hour.
Reactivity Human
Kit Size 96 well
Kit Duration 130 Minutes
Datasheets/Manuals Printable datasheet for GWB-D2CE63
Storage Refer to kit instructions for component storage
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only
Additional Information Intended Use: The Alpha 1 Microglobulin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Alpha 1 Microglobulin in biological fluid of Humans.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Alpha 1 Microglobulin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN A1M ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human A1M.
7. HUMAN A1M CALIBRATOR
One vial containing a lyophilized Human Alpha 1 Microglobulin calibrator.