Human Angiopoietin-2 ELISA Kit

GWB-SKR195

96 Wells
$479.00
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Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human Angiopoietin-2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Angiopoietin-2 proteins within the range of 94-6000 pg/ml. The Human Angiopoietin-2 ELISA is capable of recognizing both recombinant and naturally produced Human Angiopoietin-2 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference. • Human Ang-1, FGF basic (146 aa), FGF basic (157 aa), Tie-1, Tie-2, TNF-alpha, VEGF121, VEGF165 • Murine Ang-3, Tie-2

Additional Information

Name Human Angiopoietin-2 ELISA Kit
Availability In Stock
Related Product Names ANG-2
CB Replacement sc-20718; sc-7015; sc-7016; sc-7017; sc-74402; sc-74403
CB Species Human
Kit Range 94-6,000 pg/mL
Sensitivity 94 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol ANGPT2
Gene Family FIBC
Gene Fullname angiopoietin 2
Gene id 285
Alias Symbols AGPT2, Ang2, ANG2, ANG-2, Angiopoietin-2
Swissprot ID O15123
Protein Name Angiopoietin-2
Description of Target The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcri
Kit Principle The Aviva Human Angiopoietin-2 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hAngiopoietin-2 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human Angiopoietin-2 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (32 ng)
Protein Accession Num NP_001112359.1
Nucleotide Accession Num NM_001118887.1
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 94-6000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human Angiopoietin-2 ELISA is capable of recognizing both recombinant and naturally produced Human Angiopoietin-2 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference.
• Human: Ang-1, FGF basic (146 aa), FGF basic (157 aa), Tie-1, Tie-2, TNF-α, VEGF121, VEGF165
• Murine: Ang-3, Tie-2
Application ELISA
GI Number 285
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Storage 4C/6 Months
Datasheets/Manuals Printable datasheet for GWB-SKR195
Species Reactivity Human
Intended Use Research Use Only