Human ApoAI ELISA Quantitation Kit (GWB-7ACBA8)



10 x 96 Wells
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Product Name: Human Apolipoprotein (APO) AI ELISA

# of Samples: 10 X 96 Assays

Technical Features and Applications:
1. The Capture antibody should be diluted with coating buffer immediately prior to its addition to the wells. Coated plates are stable overnight at 4ºC when covered.
2. Change pipette tips between each addition of standard, sample and reagents to avoid crosscontamination.
3. Standards and samples should be pipetted to the bottom of the wells and all other reagents should be added to the side of the wells to avoid contamination.
4. Ensure that all buffers are not contaminated or expired. When troubleshooting ELISA results, it is recommended to prepare all new buffers in new vessels.
5. Do not add Sodium Azide to any of the buffers.
6. Sample and Conjugate dilutions should be made shortly before use.
7. Wash buffer should be aspirated from wells, as pouring wash buffer from wells may cause crosscontamination.
8. When preparing dilutions, wipe excess antibody/analyte from pipette tips to ensure accurate dilutions.
9. Incubation time of the Enzyme Substrate will depend on the substrate used and the intensity of the color change. The high standard should have an O.D. reading of between 1 and 3. The low standard should have an O.D. reading above background.
10. The Stopping solution should be added to the wells in the same order as the Enzyme Substrate.

Shelf life: Affinity purified IgY antibody is stable for at least two years if aliquoted andstored in non-defrosting -20oC (not -80oC) freezer. HRP-conjugated IgY canbe stored at 4C for six months or longer.

Assay Condition: The kit performance has been optimized for the stated protocol using thematerials listed and standard dilutions from 1000-1.4 ng/ml of human ApoAI.For alternative assay conditions, the operator must determine appropriatedilutions of reagents. ELISA assay reactivity is sensitive to any variation inoperator, pipetting and washing techniques, incubation time or temperature,composition or reagents, and kit age. Adjustments may be required toposition the standard curve and/or samples in the desired detection range.

Country of Origin: United States of America

Assay Use: For in vitro laboratory use only. Not for any clinical, therapeutic, ordiagnostic use in humans or animals. Not for animal or humanconsumption

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT).

Subunit: Interacts with APOA1BP (By similarity).

Subcellular Location: Secreted.

Tissue Specificity: Major protein of plasma HDL, also found in chylomicrons.

Similarity: Belongs to the apolipoprotein A1/A4/E family.

Additional Information

Name Human ApoAI ELISA Quantitation Kit (GWB-7ACBA8)
Availability In stock
Related Product Names Human ApoAI ELISA Kit; Apo-AI; ApoA-I Human ApoAI, ApoAI, Apo A1, ApoA1
Swissprot ID Q9Z2L4
Molecular Weight 30739
Datasheets/Manuals Printable datasheet for GWB-7ACBA8
Intended Use Research Use Only