Human Apolipoprotein A1 ELISA Kit (GWB-912407)

GWB-912407

40-374-130100

96 Wells
$399.00
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Additional Information

Name Human Apolipoprotein A1 ELISA Kit (GWB-912407)
Availability In Stock
Related Product Names Human Apolipoprotein A1 ELISA Kit
Kit Range 15.6 ng/ml - 500 ng/ml
Sensitivity 4.022 ng/ml
Sample Type Serum, plasma and other biological fluids
Gene Symbol APOA1
Gene Fullname apolipoprotein A1
Gene id 335
Alias Symbols Apolipoprotein A1, APOA1
Swissprot ID P02647
Protein Name Apolipoprotein A-I
Description of Target Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activat
Kit Principle Genway Biotech Apolipoprotein A1 ELISA Kit (Human) (GWB-912407) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Apolipoprotein A-I has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Apolipoprotein A-I - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apolipoprotein A-I captured in the well.
Kit Component
ComponentAmount
Anti-Human Apolipoprotein A1 Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human Apolipoprotein A1 Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Human
Standard Range Refer To Manual
Product Format In Solution
Specimen/Volumes Refer To Manual
Application ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-Apolipoprotein A-1 conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 1 hour.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-HUMAN Apolipoprotein A-1 ELISA MICRO PLATE
Anti-Human Apolipoprotein A-1 coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. HUMAN Apolipoprotein A-1 CALIBRATOR
The lyophilized Human Apolipoprotein A-1 calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 1 hour.
Reactivity Human
Kit Size 96 well
Kit Duration 100 Minutes
Storage Refer to kit instructions for component storage
Datasheets/Manuals Printable datasheet for GWB-912407
Species Reactivity Human
Intended Use Research Use Only
Additional Information Intended Use: The Apolipoprotein A-1 (ApoA-1) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring total human Apolipoprotein A-1 biological samples.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Apolipoprotein A-1 antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN Apolipoprotein A-1 ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human Apolipoprotein A-1.
7. HUMAN Apolipoprotein A-1 CALIBRATOR
One vial containing Human Apolipoprotein A-1 calibrator.