Human Betacellulin ELISA Kit (GWB-SKR006) (Aviva Cat. No. OKAG00006)

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Please see item number OKAG00006 to view product details and for ordering information.


GWB-SKR006

Aviva Cat. No.: OKAG00006

Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human Betacellulin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Betacellulin proteins within the range of 8-1000 pg/ml. The Human Betacellulin ELISA is capable of recognizing both recombinant and naturally produced Human Betacellulin proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human EGF, Epigen, Epiregulin, HB-EGF, Heregulinbeta-1, Prokinecticin-2, TGF-alpha

Additional Information

Name Human Betacellulin ELISA Kit (GWB-SKR006)
Related Product Names BTC
Kit Range 8-1,000 pg/mL
Sensitivity 8 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol BTC
Gene Fullname betacellulin
Gene id 685
Alias Symbols Probetacellulin
Swissprot ID P35070
Protein Name Probetacellulin
Description of Target This gene encodes a member of the epidermal growth factor (EGF) family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the secreted grow
Kit Principle The Aviva Human Betacellulin ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hBetacellulin concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human Betacellulin cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (100 ng)
Protein Accession Num NP_001303892.1
Nucleotide Accession Num NM_001316963.1
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 8-1000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human Betacellulin ELISA is capable of recognizing both recombinant and naturally produced Human Betacellulin proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: EGF, Epigen, Epiregulin, HB-EGF, Heregulinβ-1, Prokinecticin-2, TGF-α
Application ELISA
GI Number 685
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Datasheets/Manuals Printable datasheet for GWB-SKR006
Storage 4C/6 Months
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only