Human Carbonic Anhydrase IX ELISA Kit

GWB-SKR200

96 Wells
$479.00
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Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human Carbonic Anhydrase IX ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Carbonic Anhydrase IX proteins within the range of 32-2000 pg/ml. The Human Carbonic Anhydrase IX ELISA is capable of recognizing both recombinant and naturally produced Human Carbonic Anhydrase IX proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference. • Human Carbonic Anhydrase XII, Carbonic Anhydrase XIV • Murine Carbonic Anhydrase IX

Additional Information

Name Human Carbonic Anhydrase IX ELISA Kit
Availability In Stock
Related Product Names Carbonic anhydrase 9, Carbonate dehydratase IX, CAIX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, pMW1, G250, MN
CB Replacement sc-158316; sc-17253; sc-25599; sc-29869; sc-365900
CB Species Human
Kit Range 32-2,000 pg/mL
Sensitivity 32 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol CA9
Gene Family CA
Gene Fullname carbonic anhydrase 9
Gene id 768
Alias Symbols CAIX, CA-IX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250
Swissprot ID Q16790
Protein Name Carbonic anhydrase 9
Description of Target Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption,
Kit Principle The Aviva Human Carbonic Anhydrase IX ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hCA9 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human Carbonic Anhydrase IX cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Protein StandardLyophilized (100 ng)
Biotinylated Detection AntibodyLyophilized
400x Streptavidin-HRP30μl
Wash Buffer (15x)50 mL
Assay Diluent50 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Protein Accession Num NP_001207.2
Nucleotide Accession Num NM_001216.2
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 32-2000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human Carbonic Anhydrase IX ELISA is capable of recognizing both recombinant and naturally produced Human Carbonic Anhydrase IX proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference.
• Human: Carbonic Anhydrase XII, Carbonic Anhydrase XIV
• Murine: Carbonic Anhydrase IX
Application ELISA
GI Number 768
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Storage 4C/6 Months
Datasheets/Manuals Printable datasheet for GWB-SKR200
Species Reactivity Human
Intended Use Research Use Only