Human CRP (C-Reactive Protein) ELISA Kit (GWB-F92DE1) (Aviva Cat. No. OKIA00056)

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GWB-F92DE1

Aviva Cat. No.: OKIA00056

40-374-130034

Description

Short Description: In this assay the CRP present in sample reacts with the anti-CRP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound sample proteins by washing, anti-CRP antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound sample CRP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CRP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CRP in the test sample. The quantity of CRP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Additional Information

Name Human CRP (C-Reactive Protein) ELISA Kit (GWB-F92DE1)
Related Product Names Human CRP (C-Reactive Protein) ELISA Kit; N/A PTX1Human CRP (C-Reactive Protein)CRP
Kit Range 1.56 ng/ml - 100 ng/ml
Sensitivity 0.740 ng/ml
Sample Type Serum, plasma and other biological fluids
Gene Symbol CRP
Gene Fullname C-reactive protein
Gene id 1401
Alias Symbols CRP, PTX1
Swissprot ID P02741
Protein Name C-reactive protein
Description of Target Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may sc
Kit Principle Genway Biotech CRP ELISA Kit (Human) (GWB-F92DE1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for C-reactive protein has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-C-reactive protein - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C-reactive protein captured in the well.
Kit Component
ComponentAmount
Anti-Human CRP Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human CRP Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Human
NCBI Acc Number NP_000558.2
Standard Range Refer To Manual
Product Format In Solution
Specimen/Volumes Refer To Manual
Application ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-CRP conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-HUMAN CRP ELISA MICRO PLATE
Anti-Human CRP coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. HUMAN CRP CALIBRATOR
The lyophilized Human CRP calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Human
Kit Size 96 well
Kit Duration 40 Minutes
Datasheets/Manuals Printable datasheet for GWB-F92DE1
Storage Refer to kit instructions for component storage
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only
Additional Information Intended Use: The CRP test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring CRP in Human biological samples.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human CRP antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN CRP ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human CRP.
7. HUMAN CRP CALIBRATOR
One vial containing a lyophilized Human CRP calibrator.