Human CXCL-16 ELISA Kit (GWB-SKR011)

GWB-SKR011

96 Wells
$479.00
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Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human CXCL-16 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CXCL-16 proteins within the range of 12-3000 pg/ml. The Human CXCL-16 ELISA is capable of recognizing both recombinant and naturally produced Human CXCL-16 proteins. _x000D_ The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. _x000D_ _x000D_ • Human BRAK, ENA-78, Eotaxin, Exodus-2, Fractalkine, GRO, GRO-beta, MCP-1, MCP-2, MCP-3, MCP-4, MDC, MIG, MIP-1alpha, MIP-1beta, RANTES, SDF-1alpha, TARC_x000D_ • Murine CXCL-16, MIP-1alpha

Additional Information

Name Human CXCL-16 ELISA Kit (GWB-SKR011)
Availability In Stock
Related Product Names C-X-C motif chemokine 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, Transmembrane chemokine CXCL16, SCYB16, SRPSOX
Kit Range 12-3,000 pg/mL
Sensitivity 12 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol CXCL16
Gene Family ENDOLIG
Gene Fullname C-X-C motif chemokine ligand 16
Gene id 58191
Alias Symbols CXCLG16, C-X-C motif chemokine 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, SCYB16, Small-inducible cytokine B16, SRPSOX, SR-PSOX, Transmembrane chemokine CXCL16, UNQ2759/PRO6714
Swissprot ID Q9H2A7
Protein Name C-X-C motif chemokine 16
Description of Target Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces
Kit Principle The Aviva Human CXCL-16 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hCXCL-16 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human CXCL-16 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (100 ng)
Protein Accession Num NP_001094282.1
Nucleotide Accession Num NM_001100812.1
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 12-3000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human CXCL-16 ELISA is capable of recognizing both recombinant and naturally produced Human CXCL-16 proteins.
The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: BRAK, ENA-78, Eotaxin, Exodus-2, Fractalkine, GRO, GRO-β, MCP-1, MCP-2, MCP-3, MCP-4, MDC, MIG, MIP-1α, MIP-1β, RANTES, SDF-1α, TARC
• Murine: CXCL-16, MIP-1α
Application ELISA
GI Number 58191
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Storage 4C/6 Months
Datasheets/Manuals Printable datasheet for GWB-SKR011
Species Reactivity Human
Intended Use Research Use Only