Human CxCL10 (IP-10) ELISA (GWB-CXCL10) (Aviva Cat. No. OKIA00058)

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GWB-CXCL10

Aviva Cat. No.: OKIA00058

Additional Information

Name Human CxCL10 (IP-10) ELISA (GWB-CXCL10)
Related Product Names IP10, C-X-C motif chemokine 10, CXCL10, INP10, SCYB10, Small-inducible cytokine B10, IP-10, Gamma-IP10, 10 kDa interferon gamma-induced protein
Kit Range 0.625 ng/ml - 20 ng/ml
Sensitivity 0.176 ng/ml
Sample Type Serum, plasma and other biological fluids
Gene Symbol CXCL10
Gene Fullname C-X-C motif chemokine ligand 10
Gene id 3627
Alias Symbols IP-10, CXCL10, INP10, SCYB10, C-X-C motif chemokine 10
Swissprot ID P02778
Protein Name C-X-C motif chemokine 10
Description of Target Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
Kit Principle Genway Biotech CXCL10 ELISA Kit (Human) (GWB-CXCL10) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for C-X-C motif chemokine 10 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-C-X-C motif chemokine 10 - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C-X-C motif chemokine 10 captured in the well.
Kit Component
ComponentAmount
Anti-Human CXCL10 (IP-10) Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human CXCL10 (IP-10) Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Human
Standard Range Refer To Manual
Product Format In Solution
Specimen/Volumes Refer To Manual
Application ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-CXCL10 conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-HUMAN CXCL10 ELISA MICRO PLATE
Anti-Human CXCL10 coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. HUMAN CXCL10 CALIBRATOR
Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Human
Kit Size 96 well
Kit Duration 90 Minutes
Datasheets/Manuals Printable datasheet for GWB-CXCL10
Storage Refer to kit instructions for component storage
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only
Additional Information Intended Use: The CXCL10 test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring CXCL10 in biological samples of human.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human CXCL10 antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN CXCL10 ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human CXCL10.
7. HUMAN CXCL10 CALIBRATOR
One vial containing Human CXCL10 Calibrator.