Additional Information
| Name | Human CXCL9 (MIG) ELISA (GWB-CXCL9E) | ||||||||||||||||
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| Related Product Names | C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, HuMIG, MIG, Small-inducible cytokine B9, CXCL9, CMK, SCYB9 | ||||||||||||||||
| Kit Range | 0.313 ng/ml - 10 ng/ml | ||||||||||||||||
| Sensitivity | 0.070 ng/ml | ||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
| Gene Symbol | CXCL9 | ||||||||||||||||
| Gene Fullname | C-X-C motif chemokine ligand 9 | ||||||||||||||||
| Gene id | 4283 | ||||||||||||||||
| Alias Symbols | MIG9, CXCL9, CMK, MIG, SCYB9, C-X-C motif chemokine 9 | ||||||||||||||||
| Swissprot ID | Q07325 | ||||||||||||||||
| Protein Name | C-X-C motif chemokine 9 | ||||||||||||||||
| Description of Target | Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. | ||||||||||||||||
| Kit Principle | Genway Biotech CXCL9 ELISA Kit (Human) (GWB-CXCL9E) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for C-X-C motif chemokine 9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-C-X-C motif chemokine 9 - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C-X-C motif chemokine 9 captured in the well. | ||||||||||||||||
| Kit Component |
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| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||
| Homology | Human | ||||||||||||||||
| Standard Range | 10 ng/ml-0.313 ng/m | ||||||||||||||||
| Product Format | In Solution | ||||||||||||||||
| Specimen/Volumes | Refer To Manual | ||||||||||||||||
| Application | ELISA | ||||||||||||||||
| Reconstitution and Storage | 1. DILUENT The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C. 2. WASH SOLUTION The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C. 3. ENZYME-ANTIBODY CONJUGATE Undiluted horseradish peroxidase anti-CXCL9 conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours. 4. CHROMOGEN-SUBSTRATE SOLUTION The Substrate Solution should be stored at 4-8C and is stable until the expiration date. 5. STOP SOLUTION The Stop Solution should be stored at 4-8C and is stable until the expiration date. 6. ANTI-HUMAN CXCL9 ELISA MICRO PLATE Anti-Human CXCL9 coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack. 7. HUMAN CXCL9 CALIBRATOR Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours. |
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| Reactivity | Human | ||||||||||||||||
| Kit Size | 96 well | ||||||||||||||||
| Kit Duration | 100 Minutes | ||||||||||||||||
| Datasheets/Manuals | Printable datasheet for GWB-CXCL9E | ||||||||||||||||
| Storage | Refer to kit instructions for component storage | ||||||||||||||||
| Species Reactivity | Human | ||||||||||||||||
| Lead Time | Domestic: within 1 week delivery | International: 1 week | ||||||||||||||||
| Intended Use | Research Use Only | ||||||||||||||||
| Additional Information | Intended Use: The CXCL9 test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring CXCL9 in biological samples of human. | ||||||||||||||||
| :: | Reagents: 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 ul of affinity purified anti-Human CXCL9 antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI-HUMAN CXCL9 ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human CXCL9. 7. HUMAN CXCL9 CALIBRATOR One vial containing Human CXCL9 Calibrator. |