Human Cystatin C ELISA (GWB-374CYS)

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GWB-374CYS

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Additional Information

Name Human Cystatin C ELISA (GWB-374CYS)
Related Product Names Cystatin-3, CST3, Cystatin-C, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin, CYTC
Kit Range 0.7815 ng/ml - 50 ng/ml
Sensitivity 0.353 ng/ml
Sample Type Serum, plasma and other biological fluids
Gene Symbol CST3
Gene Fullname cystatin C
Gene id 1471
Alias Symbols Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin
Swissprot ID P01034
Protein Name Cystatin-C
Description of Target As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
Kit Principle Genway Biotech Cystatin C ELISA Kit (Human) (GWB-374CYS) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Cystatin-C has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Cystatin-C - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cystatin-C captured in the well.
Kit Component
ComponentAmount
Anti-Human Cystatin C Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human Cystatin C Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Human
NCBI Acc Number P01034
Standard Range Refer To Manual
Product Format In Solution
Specimen/Volumes Refer To Manual
Application ELISA
GI Number 118183
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-Cystatin C conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-HUMAN Cystatin C ELISA MICRO PLATE
Anti-Human Cystatin C coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. HUMAN Cystatin C CALIBRATOR
Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 10 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 1 hour after preparation.
Reactivity Human
Kit Size 96 well
Kit Duration 60 Minutes
Datasheets/Manuals Printable datasheet for GWB-374CYS
Storage Refer to kit instructions for component storage
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only
Additional Information Intended Use: The Cystatin C test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Cystatin C in human biological samples.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Cystatin C antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN Cystatin C ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human Cystatin C .
7. HUMAN Cystatin C CALIBRATOR
One vial containing Human Cystatin C calibrator.