Human Dkk-1 ELISA Kit (GWB-SKR221) (Aviva Cat. No. OKAG00221)

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Please see item number OKAG00221 to view product details and for ordering information.


GWB-SKR221

Aviva Cat. No.: OKAG00221

Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human Dkk-1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Dkk-1 proteins within the range of 63-4000 pg/ml. The Human Dkk-1 ELISA is capable of recognizing both recombinant and naturally produced Human Dkk-1 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference. • Human Dkk-4, Kremen-1, Kremen-2, LRP-6

Additional Information

Name Human Dkk-1 ELISA Kit (GWB-SKR221)
Related Product Names Dickkopf-related protein 1, Dickkopf-1
Kit Range 63-4,000 pg/mL
Sensitivity 63 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol DKK1
Gene Fullname dickkopf WNT signaling pathway inhibitor 1
Gene id 22943
Alias Symbols Dickkopf-1, Dickkopf-related protein 1, Dkk-1, DKK-1, hDkk-1, SK, UNQ492/PRO1008
Swissprot ID O94907
Protein Name Dickkopf-related protein 1
Description of Target This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marr
Kit Principle The Aviva Human Dkk-1 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hDkk-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human Dkk-1 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (53 ng)
Protein Accession Num NP_036374.1
Nucleotide Accession Num NM_012242.2
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 63-4000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human Dkk-1 ELISA is capable of recognizing both recombinant and naturally produced Human Dkk-1 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference.
• Human: Dkk-4, Kremen-1, Kremen-2, LRP-6
Application ELISA
GI Number 22943
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Datasheets/Manuals Printable datasheet for GWB-SKR221
Storage 4C/6 Months
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only