Human DPPIV ELISA Kit (GWB-SKR222) (Aviva Cat. No. OKAG00222)

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Please see item number OKAG00222 to view product details and for ordering information.


GWB-SKR222

Aviva Cat. No.: OKAG00222

Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human DPPIV ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human DPPIV proteins within the range of 32-2000 pg/ml. The Human DPP4 ELISA is capable of recognizing both recombinant and naturally produced Human DPP4 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference. • Human ACE, ACE-2, Cathepsin-A, ECE-1, ECE-2, FAP, Neprilysin • Murine DPPIV

Additional Information

Name Human DPPIV ELISA Kit (GWB-SKR222)
Related Product Names Dipeptidyl peptidase 4, ADABP, Adenosine deaminase complexing protein 2, ADCP-2, Dipeptidyl peptidase IV, DPP IV, T-cell activation antigen CD26, TP103
Kit Range 32-2,000 pg/mL
Sensitivity 32 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol DPP4
Gene Family CD
Gene Fullname dipeptidyl peptidase 4
Gene id 1803
Alias Symbols ADABP, ADCP2, ADCP-2, Adenosine deaminase complexing protein 2, CD26, Dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPPIV, DPP IV, T-cell activation antigen CD26, TP103
Swissprot ID P27487
Protein Name Dipeptidyl peptidase 4
Description of Target The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus
Kit Principle The Aviva Human DPP4 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hDPP4 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human DPP4 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (5 ng)
Protein Accession Num NP_001926.2
Nucleotide Accession Num NM_001935.3
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 32-2000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human DPP4 ELISA is capable of recognizing both recombinant and naturally produced Human DPP4 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference.
• Human: ACE, ACE-2, Cathepsin-A, ECE-1, ECE-2, FAP, Neprilysin
• Murine: DPPIV
Application ELISA
GI Number 1803
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Datasheets/Manuals Printable datasheet for GWB-SKR222
Storage 4C/6 Months
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only