Human Eotaxin-2 ELISA Kit (GWB-SKR204)

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GWB-SKR204

96 Wells
$479.00
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Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human Eotaxin-2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Eotaxin-2 proteins within the range of 32-2000 pg/ml. The Human Eotaxin-2 ELISA is capable of recognizing both recombinant and naturally produced Human Eotaxin-2 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human Eotaxin, Eotaxin-3, MIP-1 (66 aa), MIP-1 (70 aa), MCP-3 • Murine Eotaxin, Eotaxin-2

Additional Information

Name Human Eotaxin-2 ELISA Kit (GWB-SKR204)
Related Product Names C-C motif chemokine 24, CK-beta-6, Eosinophil chemotactic protein 2, Eotaxin-2, Myeloid progenitor inhibitory factor 2, Small-inducible cytokine A24, MPIF-2
Description of Target This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded
Kit Range 32-2,000 pg/mL
Sensitivity 32 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol CCL24
Gene Family CCL
Gene Fullname C-C motif chemokine ligand 24
Gene id 6369
Alias Symbols C-C motif chemokine 24, Ckb-6, CK-beta-6, Eosinophil chemotactic protein 2, eotaxin-2, Eotaxin-2, MPIF2, MPIF-2, Myeloid progenitor inhibitory factor 2, SCYA24, Small-inducible cytokine A24
Swissprot ID O00175
Protein Name C-C motif chemokine 24
Kit Principle The Aviva Human Eotaxin-2 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hEotaxin-2 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human Eotaxin-2 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (12 ng)
Protein Accession Num NP_002982.2
Nucleotide Accession Num NM_002991.2
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 32-2000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human Eotaxin-2 ELISA is capable of recognizing both recombinant and naturally produced Human Eotaxin-2 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: Eotaxin, Eotaxin-3, MIP-1 (66 aa), MIP-1 (70 aa), MCP-3
• Murine: Eotaxin, Eotaxin-2
Application ELISA
GI Number 6369
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Storage 4C/6 Months
Datasheets/Manuals Printable datasheet for GWB-SKR204
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only