Human Eotaxin-3 ELISA Kit (GWB-SKR015)

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GWB-SKR015

96 Wells
$469.00
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Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human Eotaxin-3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Eotaxin-3 proteins within the range of 32-3000 pg/ml. The Human Eotaxin-3 ELISA is capable of recognizing both recombinant and naturally produced Human Eotaxin-3 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human HCC-1, I-309, MCP-4, MIP-1alpha, MIP-1beta, MIP-4, Eotaxin, Eotaxin-2, IL-20, RANTES, Resistin, sRANK-L • Murine Eotaxin, Eotaxin-2

Additional Information

Name Human Eotaxin-3 ELISA Kit (GWB-SKR015)
Related Product Names CC chemokine IMAC, Macrophage inflammatory protein 4-alpha, MIP-4-alpha, Small-inducible cytokine A26, Thymic stroma chemokine-1, TSC-1
Kit Range 32-3,000 pg/mL
Sensitivity 32 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol CCL26
Gene Family CCL
Gene Fullname C-C motif chemokine ligand 26
Gene id 10344
Alias Symbols CC chemokine IMAC, C-C motif chemokine 26, eotaxin-3, Eotaxin-3, IMAC, Macrophage inflammatory protein 4-alpha, MGC126714, MIP-4a, MIP-4alpha, MIP-4-alpha, SCYA26, Small-inducible cytokine A26, Thymic stroma chemokine-1, TSC-1, UNQ216/PRO242
Swissprot ID Q9Y258
Protein Name C-C motif chemokine 26
Description of Target This gene is one of two Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 7. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cy
Kit Principle The Aviva Human Eotaxin-3 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hEotaxin-3 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human Eotaxin-3 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (100 ng)
Protein Accession Num NP_006063.1
Nucleotide Accession Num NM_006072.4
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 32-3000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human Eotaxin-3 ELISA is capable of recognizing both recombinant and naturally produced Human Eotaxin-3 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: HCC-1, I-309, MCP-4, MIP-1α, MIP-1β, MIP-4, Eotaxin, Eotaxin-2, IL-20, RANTES, Resistin, sRANK-L
• Murine: Eotaxin, Eotaxin-2
Application ELISA
GI Number 10344
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Datasheets/Manuals Printable datasheet for GWB-SKR015
Storage 4C/6 Months
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only