Additional Information
| Name | Human Ferritin ELISA Kit (GWB-F4BE8D) | ||||||||||||||||
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| Related Product Names | Human Ferritin ELISA Kit | ||||||||||||||||
| Kit Range | 1.56 ng/ml - 100 ng/ml | ||||||||||||||||
| Sensitivity | 0.745 ng/ml | ||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
| Gene Symbol | FTL | ||||||||||||||||
| Gene Fullname | ferritin light chain | ||||||||||||||||
| Gene id | 2512 | ||||||||||||||||
| Alias Symbols | FTL, Ferritin light chain, FTL1 | ||||||||||||||||
| Swissprot ID | P02792 | ||||||||||||||||
| Protein Name | Ferritin light chain | ||||||||||||||||
| Description of Target | Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in c | ||||||||||||||||
| Kit Principle | Genway Biotech Ferritin ELISA Kit (Human) (GWB-F4BE8D) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Ferritin light chain has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Ferritin light chain - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ferritin light chain captured in the well. | ||||||||||||||||
| Kit Component |
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| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||
| Homology | Human | ||||||||||||||||
| Standard Range | Refer To Manual | ||||||||||||||||
| Product Format | In Solution | ||||||||||||||||
| Specimen/Volumes | Refer To Manual | ||||||||||||||||
| Application | ELISA | ||||||||||||||||
| Reconstitution and Storage | 1. DILUENT The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C. 2. WASH SOLUTION The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C. 3. ENZYME-ANTIBODY CONJUGATE Undiluted horseradish peroxidase anti-Ferritin conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours. 4. CHROMOGEN-SUBSTRATE SOLUTION The Substrate Solution should be stored at 4-8C and is stable until the expiration date. 5. STOP SOLUTION The Stop Solution should be stored at 4-8C and is stable until the expiration date. 6. ANTI-HUMAN FERRITIN ELISA MICRO PLATE Anti-Human Ferritin coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack. 7. HUMAN FERRITIN CALIBRATOR Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours. |
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| Reactivity | Human | ||||||||||||||||
| Kit Size | 96 well | ||||||||||||||||
| Kit Duration | 80 Minutes | ||||||||||||||||
| Datasheets/Manuals | Printable datasheet for GWB-F4BE8D | ||||||||||||||||
| Storage | Refer to kit instructions for component storage | ||||||||||||||||
| Species Reactivity | Human | ||||||||||||||||
| Lead Time | Domestic: within 1 week delivery | International: 1 week | ||||||||||||||||
| Intended Use | Research Use Only | ||||||||||||||||
| Additional Information | Intended Use: The Ferritin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Ferritin in serum or plasma samples of humans. | ||||||||||||||||
| :: | Reagents: 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 ul of affinity purified anti-Human Ferritin antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI-HUMAN FERRITIN ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human Ferritin. 7. HUMAN FERRITIN CALIBRATOR One vial containing 1.0 ml of Human Ferritin Calibrator. |