Human Fetuin A ELISA Kit (GWB-ZZD177)

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GWB-ZZD177

96 Tests
$535.00
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Description

Short Description: Alpha2-HS glycoprotein(AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.1Human plasma protein alpha 2-HS-glycoprotein(AHSG) is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southern blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter. Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization.2

Additional Information

Name Human Fetuin A ELISA Kit (GWB-ZZD177)
Related Product Names A2HS| AHS| Ahsg| Alpha 2 HS glycoprotein| Alpha-2-HS-glycoprotein chain B| Alpha-2-Z-globulin| Ba-alpha-2-glycoprotein| FETUA| FETUA_HUMAN| Fetuin-A| HSGA
Description of Target Alpha2-HS glycoprotein (AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation
Kit Range 0.312 ng/ml - 20 ng/ml
Sensitivity < 10 pg/ml
Immunogen NSO, A19-V367
Sample Type cell culture supernates, serum or plasma (heparin, EDTA)
Kit Detection Colorimetric, OD 450 nm
Gene Symbol AHSG
Gene Fullname alpha 2-HS glycoprotein
Gene id 197
Alias Symbols Fetuin A, AHSG, FETUA
Swissprot ID P02765
Protein Name Alpha-2-HS-glycoprotein
Kit Principle Genway Biotech AHSG ELISA Kit (Human) (GWB-ZZD177) is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody specific for AHSG has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (NSO, A19-V367) and test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AHSG is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Human AHSG captured in well.
Kit Component
ComponentAmount
Lyophilized recombinant mouse C-MET standard10 ng/tube x 2
Anti-mouse C-MET Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti- mouse C-MET antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Protein Accession Num NP_001613.2
Nucleotide Accession Num NM_001622.2
Homology No detectable cross-reactivity with any other cytokine.
Standard Range 312pg/ml-20,000pg/ml. Test has a sensitivity of < 10 pg/ml
Specimen/Volumes Refer to Manual
Specificity There is no detectable cross-reactivity with other relevant proteins.
Concentration Lot Specific
Application ELISA
GI Number 197
Stability Four months at 4C and eight months at -20C.
Reconstitution and Storage Store at 4C for frequent use, at  -20C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Reactivity Reactive to Human. There is no detectable cross-reactivity with other relevant proteins.
Kit Size 1x96 well plate
Kit Duration ~ 3 Hours
Storage Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Datasheets/Manuals Printable datasheet for GWB-ZZD177
Species Reactivity Human
Intended Use Research Use Only
Key Reference 11. Osawa, M., Tian, W., Horiuchi, H., Kaneko, M., Umetsu, K. Association of alpha-2-HS glycoprotein (AHSG, fetuin-A)
polymorphism with AHSG and phosphate serum levels. Hum. Genet. 116: 146-151, 2005.
2. Magnuson, V. L., McCombs, J. L., Lee, C.-C., Yang, F., Bowman, B. H., McGill, J. R. Human alpha-2-HS-glycoprotein
localized to 3q27-q29 by in situ hybridization. Cytogenet. Cell Genet. 47: 72-74, 1988.
Additional Information Range: 0.312ng/ml => 20ng/ml
:: Principle: Aviva’s Human Fetuin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Fetuin A specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Fetuin A amount of sample captured in plate.
:: Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before using.
Background: Alpha2-HS glycoprotein (AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.1Human plasma protein alpha 2-HS-glycoprotein (AHSG) is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southern blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter (Lee et al., 1987). Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization.2