Human Flt-3 Ligand ELISA Kit (GWB-SKR123)

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GWB-SKR123

96 Wells
$469.00
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Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human Flt-3 Ligand ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Flt-3 Ligand proteins within the range of 16-1000 pg/ml. The Human Flt-3L ELISA is capable of recognizing both recombinant and naturally produced Human Flt-3L proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human FGF-5, Flt-1/Fc, IL-8, PlGF, TNF-alpha, VEGF121, VEGF165 • Murine Flt-3L, IL-5

Additional Information

Name Human Flt-3 Ligand ELISA Kit (GWB-SKR123)
Related Product Names Fms-related tyrosine kinase 3 ligand, SL cytokine
Kit Range 16-1,000 pg/mL
Sensitivity 16 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol FLT3LG
Gene Family ENDOLIG
Gene Fullname fms related tyrosine kinase 3 ligand
Gene id 2323
Alias Symbols FL, Flt3L, FLT3L, Flt3 ligand, Fms-related tyrosine kinase 3 ligand, SL cytokine
Swissprot ID P49771
Protein Name Fms-related tyrosine kinase 3 ligand
Description of Target Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see
Kit Principle The Aviva Human Flt-3 Ligand ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hFlt-3L concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human Flt-3 Ligand while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetricreaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (8.5 ng)
Protein Accession Num NP_001191431.1
Nucleotide Accession Num NM_001204502.1
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 16-1000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human Flt-3L ELISA is capable of recognizing both recombinant and naturally produced Human Flt-3L proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: FGF-5, Flt-1/Fc, IL-8, PlGF, TNF-α, VEGF121, VEGF165
• Murine: Flt-3L, IL-5
Application ELISA
GI Number 2323
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Datasheets/Manuals Printable datasheet for GWB-SKR123
Storage 4C/6 Months
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only