Human Galectin-3 ELISA Kit (GWB-SKR226)

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GWB-SKR226

96 Wells
$479.00
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Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human Galectin-3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Galectin-3 proteins within the range of 63-4000 pg/ml. The Human Galectin-3 ELISA is capable of recognizing both recombinant and naturally produced Human Galectin-3 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference. • Human Galectin-1, Galectin-2, Galectin-4, Galectin-7, Galectin-8, Galectin-10

Additional Information

Name Human Galectin-3 ELISA Kit (GWB-SKR226)
Related Product Names Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, IgE-binding protein, Laminin-binding protein, Lectin L-29, Mac-2 antigen
Description of Target This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single
Kit Range 63-4,000 pg/mL
Sensitivity 63 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol LGALS3
Gene Family ENDOLIG
Gene Fullname lectin, galactoside binding soluble 3
Gene id 3958
Alias Symbols 35 kDa lectin, Carbohydrate-binding protein 35, CBP35, CBP 35, GAL3, Gal-3, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, Galectin-3, GALIG, IgE-binding protein, L31, L-31, Laminin-binding protein, Lectin L-29, LGALS2, MAC2, MAC-2, Mac-
Swissprot ID P17931
Protein Name Galectin-3
Kit Principle The Aviva Human Galectin-3 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hGal-3 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human Galectin-3 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (17 ng)
Protein Accession Num NP_002297.2
Nucleotide Accession Num NM_002306.3
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 63-4000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human Galectin-3 ELISA is capable of recognizing both recombinant and naturally produced Human Galectin-3 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference.
• Human: Galectin-1, Galectin-2, Galectin-4, Galectin-7, Galectin-8, Galectin-10
Application ELISA
GI Number 3958
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Storage 4C/6 Months
Datasheets/Manuals Printable datasheet for GWB-SKR226
Species Reactivity Human
Intended Use Research Use Only