Human GCP-2 ELISA Kit (GWB-SKR219) (Aviva Cat. No. OKAG00219)

This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKAG00219 to view product details and for ordering information.


GWB-SKR219

Aviva Cat. No.: OKAG00219

Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human GCP-2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human GCP-2 proteins within the range of 16-1000 pg/ml. The Human GCP-2 ELISA is capable of recognizing both recombinant and naturally produced Human GCP-2 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference. • Human BLC, ENA-78, GROalpha, GRObeta, IL-8, IP-10, MIG, NAP-2, SDF-1alpha, SDF-1beta • Murine GCP-2, MIP-2

Additional Information

Name Human GCP-2 ELISA Kit (GWB-SKR219)
Related Product Names Chemokine alpha 3, CKA-3, Granulocyte chemotactic protein 2, GCP-2, Small-inducible cytokine B6
Kit Range 16-1,000 pg/mL
Sensitivity 16 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol CXCL6
Gene Family ENDOLIG
Gene Fullname C-X-C motif chemokine ligand 6
Gene id 6372
Alias Symbols Chemokine alpha 3, CKA-3, C-X-C motif chemokine 6, GCP2, GCP-2, Granulocyte chemotactic protein 2, SCYB6, Small-inducible cytokine B6
Swissprot ID P80162
Protein Name C-X-C motif chemokine 6
Description of Target Chemotactic for neutrophil granulocytes. Signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bact
Kit Principle The Aviva Human GCP-2 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hGCP-2 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human GCP-2 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (17 ng)
Protein Accession Num NP_002984.1
Nucleotide Accession Num NM_002993.3
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 16-1000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human GCP-2 ELISA is capable of recognizing both recombinant and naturally produced Human GCP-2 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference.
• Human: BLC, ENA-78, GROα, GROβ, IL-8, IP-10, MIG, NAP-2, SDF-1α, SDF-1β
• Murine: GCP-2, MIP-2
Application ELISA
GI Number 6372
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Datasheets/Manuals Printable datasheet for GWB-SKR219
Storage 4C/6 Months
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only