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Human I-TAC ELISA Kit (GWB-SKR046)

This exact product is now provided by Aviva Systems Biology.
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GWB-SKR046

96 Wells
$479.00
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Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human I-TAC ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human I-TAC proteins within the range of 32-2000 pg/ml. The Human I-TAC ELISA is capable of recognizing both recombinant and naturally produced Human I-TAC proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity. • Murine I-TAC The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human ENA-78, IL-2, IL-8 (72 aa), IL-8 (77 aa), IP-10, MIG, NAP-2

Additional Information

Name Human I-TAC ELISA Kit (GWB-SKR046)
Related Product Names C-X-C motif chemokine 11, Beta-R1, H174, Interferon gamma-inducible protein 9, Interferon-inducible T-cell alpha chemoattractant, Small-inducible cytokine B11, SCYB11, SCYB9B
Description of Target Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chem
Kit Range 32-2,000 pg/mL
Sensitivity 32 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol CXCL11
Gene Family ENDOLIG
Gene Fullname C-X-C motif chemokine ligand 11
Gene id 6373
Alias Symbols Beta-R1, b-R1, C-X-C motif chemokine 11, H174, Interferon gamma-inducible protein 9, Interferon-inducible T-cell alpha chemoattractant, IP9, IP-9, ITAC, I-TAC, MGC102770, SCYB11, SCYB9B, Small-inducible cytokine B11
Swissprot ID O14625
Protein Name C-X-C motif chemokine 11
Kit Principle The Aviva Human I-TAC ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hI-TAC concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human I-TAC cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (100 ng)
Protein Accession Num NP_001289052.1
Nucleotide Accession Num NM_001302123.1
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 32-2000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human I-TAC ELISA is capable of recognizing both recombinant and naturally produced Human I-TAC proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity.
• Murine: I-TAC
The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: ENA-78, IL-2, IL-8 (72 aa), IL-8 (77 aa), IP-10, MIG, NAP-2
Application ELISA
GI Number 6373
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Storage 4C/6 Months
Datasheets/Manuals Printable datasheet for GWB-SKR046
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only