Description
Short Description: In this assay the IgE present in serum sample reacts with the anti-IgE antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound serum proteins by washing, anti-IgE antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound serum IgE. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgE in the test sample. The quantity of IgE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for serum dilution.
Additional Information
| Name | Human IgE ELISA Kit (GWB-2E2ECD) | ||||||||||||||||
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| Related Product Names | Human IgE ELISA Kit; N/A Human IgEIGHE | ||||||||||||||||
| Kit Range | 3.125 ng/ml - 200 ng/ml | ||||||||||||||||
| Sensitivity | 0.912 ng/ml | ||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
| Alias Symbols | IGHE, Ig epsilon chain C region, IgE | ||||||||||||||||
| Swissprot ID | P01854 | ||||||||||||||||
| Protein Name | Immunoglobulin heavy constant epsilon | ||||||||||||||||
| Description of Target | Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as re | ||||||||||||||||
| Kit Principle | Genway Biotech IgE ELISA Kit (Human) (GWB-2E2ECD) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Immunoglobulin heavy constant epsilon has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Immunoglobulin heavy constant epsilon - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Immunoglobulin heavy constant epsilon captured in the well. | ||||||||||||||||
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| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||
| Homology | Human | ||||||||||||||||
| Standard Range | Refer To Manual | ||||||||||||||||
| Product Format | In Solution | ||||||||||||||||
| Specimen/Volumes | Refer To Manual | ||||||||||||||||
| Application | ELISA | ||||||||||||||||
| Reconstitution and Storage | 1. DILUENT The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C. 2. WASH SOLUTION The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C. 3. ENZYME-ANTIBODY CONJUGATE Undiluted horseradish peroxidase anti-IgE conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours. 4. CHROMOGEN-SUBSTRATE SOLUTION The Substrate Solution should be stored at 4-8C and is stable until the expiration date. 5. STOP SOLUTION The Stop Solution should be stored at 4-8C and is stable until the expiration date. 6. ANTI-HUMAN IgE ELISA MICRO PLATE Anti-Human IgE coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack. 7. HUMAN IgE CALIBRATOR Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours. |
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| Reactivity | Human | ||||||||||||||||
| Kit Size | 96 well | ||||||||||||||||
| Kit Duration | 80 Minutes | ||||||||||||||||
| Datasheets/Manuals | Printable datasheet for GWB-2E2ECD | ||||||||||||||||
| Storage | Refer to kit instructions for component storage | ||||||||||||||||
| Species Reactivity | Human | ||||||||||||||||
| Lead Time | Domestic: within 1 week delivery | International: 1 week | ||||||||||||||||
| Intended Use | Research Use Only | ||||||||||||||||
| Additional Information | Intended Use: The total Human IgE test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgE in human biological samples. | ||||||||||||||||
| :: | Reagents: 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 ul of affinity purified anti-Human IgE antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI-HUMAN IgE ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human IgE. 7. HUMAN IgE CALIBRATOR One vial containing of Human IgE calibrator. |