Human IgG ELISA Kit (GWB-A04A50) (Aviva Cat. No. OKIA00070)

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GWB-A04A50

Aviva Cat. No.: OKIA00070

40-374-130037

Description

Short Description: In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Additional Information

Name Human IgG ELISA Kit (GWB-A04A50)
Related Product Names Human IgG ELISA Kit; N/A Human IgGIGHG1
Kit Range 15.6 ng/ml - 500 ng/ml
Sensitivity 1.816 ng/ml
Sample Type Serum, plasma and other biological fluids
Alias Symbols IGHG1, Ig gamma, Immunoglobulin G, Ig gamma-1 chain C region
Swissprot ID P01857
Protein Name Immunoglobulin heavy constant gamma 1
Description of Target Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as re
Kit Principle Genway Biotech IgG ELISA Kit (Human) (GWB-A04A50) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Immunoglobulin heavy constant gamma 1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Immunoglobulin heavy constant gamma 1 - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Immunoglobulin heavy constant gamma 1 captured in the well.
Kit Component
ComponentAmount
Anti-Human IgG Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human IgG Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Human
Standard Range Refer To Manual
Product Format In Solution
Specimen/Volumes Refer To Manual
Application ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-IgG conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-HUMAN IgG ELISA MICRO PLATE
Anti-Human IgG coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. HUMAN IgG CALIBRATOR
Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Human
Kit Size 96 well
Kit Duration 90 Minutes
Datasheets/Manuals Printable datasheet for GWB-A04A50
Storage Refer to kit instructions for component storage
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only
Additional Information Intended Use: The total Human IgG test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgG in Human Biological Samples.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human IgG antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN IgG ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human IgG.
7. HUMAN IgG CALIBRATOR
One vial containing Human IgG calibrator.