Human IL-18 BPa ELISA Kit (GWB-SKR140) (Aviva Cat. No. OKAG00140)

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GWB-SKR140

Aviva Cat. No.: OKAG00140

Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human IL-18 BPa ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-18 BPa proteins within the range of 94-6000 pg/ml. The Human IL-18 BPa ELISA is capable of recognizing both recombinant and naturally produced Human IL-18 BPa proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human IL-18 BPc, IL-18BPd

Additional Information

Name Human IL-18 BPa ELISA Kit (GWB-SKR140)
Related Product Names Interleukin-18-binding protein, Tadekinig-alfa
Kit Range 94-6,000 pg/mL
Sensitivity 94 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol IL18BP
Gene Family IGD
Gene Fullname interleukin 18 binding protein
Gene id 10068
Alias Symbols IL-18BP, IL18BPa, Interleukin-18-binding protein, Tadekinig-alfa
Swissprot ID O95998
Protein Name Interleukin-18-binding protein
Description of Target The protein encoded by this gene functions as an inhibitor of the proinflammatory cytokine, IL18. It binds IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production, resulting in reduced T-helper type 1 immune
Kit Principle The Aviva Human IL-18 BPa ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hIL-18 BPa concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator.The capture antibodies coated to the bottom of each well are specific for a particular epitope on theHuman IL-18 BPa cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetricreaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (33 ng)
Protein Accession Num NP_001034748.1
Nucleotide Accession Num NM_001039659.1
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 94-6000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human IL-18 BPa ELISA is capable of recognizing both recombinant and naturally produced Human IL-18 BPa proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: IL-18 BPc, IL-18BPd
Application ELISA
GI Number 10068
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Datasheets/Manuals Printable datasheet for GWB-SKR140
Storage 4C/6 Months
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only