Human IL-3 ELISA Kit (GWB-SKR036)

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96 Wells
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Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human IL-3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-3 proteins within the range of 16-4000 pg/ml. The Human IL-3 ELISA is capable of recognizing both recombinant and naturally produced Human IL-3 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human G-CSF, GM-CSF, IL-5, IL-7, TPO • Murine IL-3 • Rat IL-3beta

Additional Information

Name Human IL-3 ELISA Kit (GWB-SKR036)
Related Product Names Interleukin-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF
Kit Range 16-4,000 pg/mL
Sensitivity 16 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol IL3
Gene Family IL
Gene Fullname interleukin 3
Gene id 3562
Alias Symbols Hematopoietic growth factor, IL-3, Interleukin-3, Mast cell growth factor, MCGF, MGC79398, MGC79399, MULTI-CSF, Multipotential colony-stimulating factor, P-cell-stimulating factor
Swissprot ID P08700
Protein Name Interleukin-3
Description of Target The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiat
Kit Principle The Aviva Human IL-3 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hIL-3 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human IL-3 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (100 ng)
Protein Accession Num NP_000579.2
Nucleotide Accession Num NM_000588.3
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 16-4000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human IL-3 ELISA is capable of recognizing both recombinant and naturally produced Human IL-3 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: G-CSF, GM-CSF, IL-5, IL-7, TPO
• Murine: IL-3
• Rat: IL-3β
Application ELISA
GI Number 3562
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Datasheets/Manuals Printable datasheet for GWB-SKR036
Storage 4C/6 Months
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only