Human MIP-1alpha ELISA Kit (GWB-SKR052)

This exact product is now provided by Aviva Systems Biology.
Please visit OKAG00052 to review further and for ordering information.


GWB-SKR052

96 Wells
$469.00

Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human MIP-1alpha ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human MIP-1alpha proteins within the range of 16-3000 pg/ml. The Human MIP-1alpha ELISA is capable of recognizing both recombinant and naturally produced Human MIP-1alpha proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human IP-10, MCP-1, MIP-1beta, MIP-3, MIP-3alpha, MIP-3beta, RANTES • Murine MIP-1alpha, MIP-1beta, MIP-1gamma, MIP-3alpha, MIP-3beta • Rat MIP-1alpha

Additional Information

Name Human MIP-1alpha ELISA Kit (GWB-SKR052)
Related Product Names C-C motif chemokine 3, G0/G1 switch regulatory protein 19-1, Macrophage inflammatory protein 1-alpha, PAT 464.1, SIS-beta, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein, SCYA3, G0S19-1
Kit Range 16-3,000 pg/mL
Sensitivity 16 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol CCL3
Gene Family CCL
Gene Fullname C-C motif chemokine ligand 3
Gene id 6348
Alias Symbols C-C motif chemokine 3, G0/G1 switch regulatory protein 19-1, G0S19-1, LD78ALPHA, Macrophage inflammatory protein 1-alpha, MIP1A, MIP-1-alpha, PAT 464.1, SCYA3, SIS-beta, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein
Swissprot ID P10147
Protein Name C-C motif chemokine 3
Description of Target This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be a
Kit Principle The Aviva Human MIP-1alpha ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hMIP-1alpha concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human MIP-1alpha cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (100 ng)
Protein Accession Num NP_002974.1
Nucleotide Accession Num NM_002983.2
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 16-3000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human MIP-1α ELISA is capable of recognizing both recombinant and naturally produced Human MIP-1α proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: IP-10, MCP-1, MIP-1β, MIP-3, MIP-3α, MIP-3β, RANTES
• Murine: MIP-1α, MIP-1β, MIP-1γ, MIP-3α, MIP-3β
• Rat: MIP-1α
Application ELISA
GI Number 6348
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Datasheets/Manuals Printable datasheet for GWB-SKR052
Storage 4C/6 Months
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only