Human PAI-1 ELISA Kit (GWB-SKR055)

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GWB-SKR055

96 Wells
$479.00
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Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human PAI-1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human PAI-1 proteins within the range of 23-3000 pg/ml. The Human PAI-1 ELISA is capable of recognizing both recombinant and naturally produced Human PAI-1 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human Neuroserpin, Maspin, PAI-2, PEDF, Vaspin

Additional Information

Name Human PAI-1 ELISA Kit (GWB-SKR055)
Related Product Names Plasminogen activator inhibitor 1, Endothelial plasminogen activator inhibitor, Serpin E1, PLANH1
Description of Target This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the c
Kit Range 23-3,000 pg/mL
Sensitivity 23 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol SERPINE1
Gene Family SERPIN
Gene Fullname serpin family E member 1
Gene id 5054
Alias Symbols Endothelial plasminogen activator inhibitor, PAI, PAI1, PAI-1, PLANH1, Plasminogen activator inhibitor 1, Serpin E1
Swissprot ID P05121
Protein Name Plasminogen activator inhibitor 1
Kit Principle The Aviva Human PAI-1 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hPAI-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human PAI-1 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (100 ng)
Protein Accession Num NP_000593.1
Nucleotide Accession Num NM_000602.4
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 23-3000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human PAI-1 ELISA is capable of recognizing both recombinant and naturally produced Human PAI-1 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: Neuroserpin, Maspin, PAI-2, PEDF, Vaspin
Application ELISA
GI Number 5054
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Storage 4C/6 Months
Datasheets/Manuals Printable datasheet for GWB-SKR055
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only