Human Progranulin ELISA Kit (GWB-SKR243)

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GWB-SKR243

96 Wells
$479.00
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Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human Progranulin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Progranulin proteins within the range of 63-4000 pg/ml. The Human Progranulin ELISA is capable of recognizing both recombinant and naturally produced Human Progranulin proteins. The antigens listed below were tested at 60 ng/ml and exhibited less than 2% cross reactivity. • Murine Progranulin The antigens listed below were tested at 120 ng/ml and did not exhibit significant cross reactivity or interference. • Human N-Cadherin, P-Cadherin, VProgranulin • Murine P-Cadherin

Additional Information

Name Human Progranulin ELISA Kit (GWB-SKR243)
Related Product Names Granulins, Proepithelin, PEPI
Description of Target Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin
Kit Range 63-4,000 pg/mL
Sensitivity 63 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol GRN
Gene Fullname granulin
Gene id 2896
Alias Symbols GEP, GP88, Granulins, PCDGF, PEPI, PGRN, Proepithelin
Swissprot ID P28799
Protein Name Granulins
Kit Principle The Aviva Human Progranulin ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hProgranulin concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human Progranulin cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (12 ng)
Protein Accession Num NP_002078.1
Nucleotide Accession Num NM_002087.3
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 63-4000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human Progranulin ELISA is capable of recognizing both recombinant and naturally produced Human Progranulin proteins. The antigens listed below were tested at 60 ng/ml and exhibited less than 2% cross reactivity.
• Murine: Progranulin
The antigens listed below were tested at 120 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: N-Cadherin, P-Cadherin, VProgranulin
• Murine: P-Cadherin
Application ELISA
GI Number 2896
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Storage 4C/6 Months
Datasheets/Manuals Printable datasheet for GWB-SKR243
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only