Human SDF-1 ELISA Kit (GWB-SKR216) (Aviva Cat. No. OKAG00216)

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Please see item number OKAG00216 to view product details and for ordering information.


GWB-SKR216

Aviva Cat. No.: OKAG00216

Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human SDF-1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human SDF-1 proteins within the range of 32-2000 pg/ml. The Human SDF-1 ELISA is capable of recognizing both recombinant and naturally produced Human SDF-1 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference. • Human IP-10/CRG-2 • Murine IP-10/CRG-2

Additional Information

Name Human SDF-1 ELISA Kit (GWB-SKR216)
Related Product Names Stromal cell-derived factor 1 , C-X-C motif chemokine 12, Intercrine reduced in hepatomas, IRH, Pre-B cell growth-stimulating factor, PBSF
Kit Range 32-2,000 pg/mL
Sensitivity 32 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol CXCL12
Gene Family ENDOLIG
Gene Fullname C-X-C motif chemokine ligand 12
Gene id 6387
Alias Symbols C-X-C motif chemokine 12, hIRH, hSDF-1, Intercrine reduced in hepatomas, IRH, PBSF, Pre-B cell growth-stimulating factor, SCYB12, SDF1, SDF-1, SDF1A, SDF-1a, SDF1B, SDF-1b, Stromal cell-derived factor 1, TLSF, TLSF-a, TLSF-b, TPAR1
Swissprot ID P48061
Protein Name Stromal cell-derived factor 1
Description of Target This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse ce
Kit Principle The Aviva Human SDF-1 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human SDF-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human SDF-1 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Protein StandardLyophilized (4.4 ng)
Biotinylated Detection AntibodyLyophilized
400x Streptavidin-HRP30μl
Wash Buffer (15x)50 mL
Assay Diluent50 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Protein Accession Num NP_000600.1
Nucleotide Accession Num NM_000609.6
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 32-2000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human SDF-1 ELISA is capable of recognizing both recombinant and naturally produced Human SDF-1 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference.
• Human: IP-10/CRG-2
• Murine: IP-10/CRG-2
Application ELISA
GI Number 6387
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Datasheets/Manuals Printable datasheet for GWB-SKR216
Storage 4C/6 Months
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only