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Human TNF alpha ELISA Kit (GWB-ZZD003)


96 wells
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Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

Additional Information

Name Human TNF alpha ELISA Kit (GWB-ZZD003)
Related Product Names APC1 protein| Cachectin| DIF| soluble form| Tnf| TNF a| TNF alpha| TNF macrophage derived| TNF monocyte derived| TNF superfamily member 2| TNF-a| TNF-alpha| TNFA_HUMAN| TNFSF2| Tumor necrosis factor| Tumor necrosis factor alpha| Tumor necrosis factor li
Short Description Sandwich ELISA kit of Quantitative Detection for Human TNF alpha
Sample Type This kit is used for quantitative detection of human TNF alpha in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Swissprot ID P01375
Immunogen please inquire
Standard Range 15.6pg/ml-1000pg/ml(cell culture supernates), 7.8pg/ml-500pg/ml(serum, plasma). Test has a sensitivity of < 1 pg/ml
Specimen/Volumes Refer to Manual
Concentration Lot Specific
Application ELISA
GI Number 7124
Stability Four months at 4C and eight months at -20C.
Reactivity Reactive to Human. There is no detectable cross-reactivity with other relevant proteins.
Kit Size 1x96 well plate
Datasheets/Manuals Printable datasheet for GWB-ZZD003
Storage Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Intended Use Research Use Only