Human VEGF-R1 ELISA Kit (GWB-SKR176) (Aviva Cat. No. OKAG00176)

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GWB-SKR176

Aviva Cat. No.: OKAG00176

Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human VEGF-R1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human VEGF-R1 proteins within the range of 157-10000 pg/ml. The Human VEGF-R1 ELISA is capable of recognizing both recombinant and naturally produced Human VEGF-R1 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human PIGF, VEGF121, VEGF165, VEGF/PIGF, VEGF 42/Fc Chimera, VEGF R3/Fc Chimera • Murine VEGF R1 (Flt-1)/Fc Chimera

Additional Information

Name Human VEGF-R1 ELISA Kit (GWB-SKR176)
Related Product Names Vascular endothelial growth factor receptor 1, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor, FLT, FRT
Kit Range 157-1,0000 pg/mL
Sensitivity 157 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol FLT1
Gene Family IGD
Gene Fullname fms related tyrosine kinase 1
Gene id 2321
Alias Symbols FLT, FLT-1, Fms-like tyrosine kinase 1, FRT, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular endothelial growth factor receptor 1, Vascular permeability factor receptor, VEGFR1, VEGFR-1
Swissprot ID P17948
Protein Name Vascular endothelial growth factor receptor 1
Description of Target This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a tra
Kit Principle The Aviva Human VEGF-R1 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hVEGF-R1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human VEGF-R1 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (37.5 ng)
Protein Accession Num NP_001153392.1
Nucleotide Accession Num NM_001159920.1
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 157-10000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human VEGF-R1 ELISA is capable of recognizing both recombinant and naturally produced Human VEGF-R1 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: PIGF, VEGF121, VEGF165, VEGF/PIGF, VEGF 42/Fc Chimera, VEGF R3/Fc Chimera
• Murine: VEGF R1 (Flt-1)/Fc Chimera
Application ELISA
GI Number 2321
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Datasheets/Manuals Printable datasheet for GWB-SKR176
Storage 4C/6 Months
Species Reactivity Human
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only