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HSV-2 IgG Enzyme Immunoassay Test Kit



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Species: Human

Sample Type: Serum

For the quantitative determination of IgG Antibodies to Herpes Simplex Virus Type 2 (HSV-2) in serum. DNA Virus Infections, Infection, Blood, Female Urogenital Diseases, Sexually Transmitted Diseases

Product Name: IgG Antibodies to Herpes Simplex Virus Type 2 (HSV-2)

Sample Type: Serum

# of Samples: 1X96 Assays

Intended Use: The HSV-2 IgG ELISA is intended for use in evaluating a patient\'s serologic status to herpes simplex virus (HSV) type 2 infection, or for evaluating paired sera for the presence of a significant increase in herpes specific IgG.

Introduction: Herpes Simplex Virus is a common pathogen and its primary infection is usually asymptomatic. There are two immunologically distinct types of HSV: Type 1 and Type 2. HSV-1 is generally associated with oral infection and lesions above the waist, and HSV-2 is associated with genital infections and lesions below the waist. Clinical cases primarily are (1) eczema herpeticum with eczematous skin changes with numerous lesions, (2) gingivostomatitis, and (3) herpes sepsis, almost only found in premature newborn infants. The GenWay HSV-2 IgG ELISA is an accurate serologic method to detect HSV-2 IgG specific-antibody in serum samples.

Principle of the test: Purified HSV-2 antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the HSV-2 IgG-specific antibody, if present, binds to the antigen. All unbound materials are washed away. HRP-conjugate is added, which binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and a solution of TMB Reagent is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of HSV-2 IgG-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.

Storage and Stability: 1. Store the kit at 2-8C.
2. Keep microwells sealed in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit.
4. Do not expose test reagents to heat, sun or strong light during storage or usage.

Limitation of the Test: 1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the package insert instructions and with adherence to good laboratory practice.
2. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings.
3. Serum samples demonstrating gross lipemia, gross hemolysis, or turbidity should not be used with this test.
4. The results obtained from the use of this kit should be used only as an adjunct to other diagnostic procedures and information available to the physician.

Product Name: IgG Antibodies to Herpes Simplex Virus Type 2 (HSV-2)

Subcellular Location:

Disease: Chromosomal aberrations involving IGHG1 may be a cause of multiple myeloma [MIM:254500]. Translocation t(11;14)(q13;q32) with CCND1; translocation t(4;14)(p16.3;q32.3) with FGFR3; translocation t(6;14)(p25;q32) with IRF4.

Miscellaneous: Nie has the G1M(17) allotypic marker, 97-K, and the G1M(1) markers, 239-D and 241-L. KOL and EU sequences have the G1M(3) marker and the G1M (non-1) markers.

Miscellaneous: Nie also differs in the amidation states of 35, 116, 198, 269 and 272.

Miscellaneous: EU also differs in the amidation states of residues 155, 166, 177, 195, 198, 269, and 272 and in the order of residues 268-272.

Miscellaneous: KOL also differs in the amidation states of residues 198, 267 and 272.

Additional Information

Name HSV-2 IgG Enzyme Immunoassay Test Kit
Related Product Names IgG Antibodies to Herpes Simplex Virus Type 2 (HSV-2) ELISA Kit; N/A IgG Antibodies to Herpes Simplex Virus Type 2 (HSV-2)HSV-2
Swissprot ID P01857
Molecular Weight 36106
Datasheets/Manuals Printable manual for GWB-5185FE
Intended Use Research Use Only