Toxoplasma IgG Enzyme Immunoassay Test Kit (GWB-789E36)



96 Wells
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Species: Human

Sample Type: Serum

For the quantitative determination of IGG Antibodies to Toxoplasma gondii in serum. Protozoan Infections, Infection, Blood, Central Nervous System Diseases, Central Nervous System Infections

Product Name:
IGG Antibodies to Toxoplasma gondii

Sample Type: Serum

# of Samples: 1X96 Assays

Intended Use: The GenWay Biotech Toxoplasma IgG ELISA is intended for use in evaluating serologic status to Toxoplasma gondii infection.

Introduction: Toxoplasmosis is caused by the intracellular parasite Toxoplasma gondii and may be contracted by consuming contaminated meat or by coming in contact with cat feces containing oocysts. In adolescence and adulthood, most infections are subclinical. However, if a pregnant woman contracts toxoplasmosis, it may be passed through the placenta to the fetus, resulting in congenital toxoplasmosis, which is a cause of mortality and malformation. Asymptomatic infants may develop abnormalities later in life.

Principle of the test: Purified Toxoplasma gondii antigen is coated on the surface of microwells. Diluted serum is added to the wells, and the Toxoplasma gondii IgG-specific antibody, if present, binds to the antigen. All unbound materials are washed away. HRP-conjugate is added, which binds to the antibody-antigen complex. Excess HRP-conjugate is washed off and a solution of TMB Reagent is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgG-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.

Limitation of the Test: 1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding
of the package insert instructions and with adherence to good laboratory practice.
2. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings.
3. Serum samples demonstrating gross lipemia, gross hemolysis, or turbidity should not be used with this test.

Precision: A study was performed to document typical assay precision with the GenWay Biotech Toxo IgG EIA product. The mean, SD, and %CV were calculated for Intra- and Inter-Assay in concentration value (IU/mL).

Product Name: IGG Antibodies to Toxoplasma gondii

Subcellular Location:

Disease: Chromosomal aberrations involving IGHG1 may be a cause of multiple myeloma [MIM:254500]. Translocation t(11;14)(q13;q32) with CCND1; translocation t(4;14)(p16.3;q32.3) with FGFR3; translocation t(6;14)(p25;q32) with IRF4.

Miscellaneous: Nie has the G1M(17) allotypic marker, 97-K, and the G1M(1) markers, 239-D and 241-L. KOL and EU sequences have the G1M(3) marker and the G1M (non-1) markers.

Miscellaneous: Nie also differs in the amidation states of 35, 116, 198, 269 and 272.

Miscellaneous: EU also differs in the amidation states of residues 155, 166, 177, 195, 198, 269, and 272 and in the order of residues 268-272.

Miscellaneous: KOL also differs in the amidation states of residues 198, 267 and 272.

Additional Information

Name Toxoplasma IgG Enzyme Immunoassay Test Kit (GWB-789E36)
Related Product Names IgG Antibodies to Toxoplasma gondii ELISA Kit;
Swissprot ID P01857
Molecular Weight 36106
Datasheets/Manuals Printable datasheet for GWB-789E36
Intended Use Research Use Only